How to Calculate Km From Lineweaver Burk Plot

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Lineweaver Burk Plot Mcat Chemistry Equation

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. According to the Lineweaver-Burk plot the Vmax is reduced during the addition of a non-competitive inhibitor which is shown in the plot by a change in both the slope and y-intercept when a non-competitive inhibitor is added. You can measure product formation using several inhibitor concentrations extract the data create your own Lineweaver-Burk plot and solve the Ki. When a non-competitive inhibitor is added the Vmax is changed while the Km remains unchanged.

With the newly added module of enzyme inhibition you are asked to perform different enzyme inhibition experiments using three different inhibitors. Wishing for a unique insight into a subject matter for your subsequent individual research.

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